Review



pe cy7 conjugated anti mouse rat bcl 2  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Thermo Fisher pe cy7 conjugated anti mouse rat bcl 2
    (A) WT B6 mice were left undisturbed or restrained for 12 h, after which HMNCs and splenocytes were harvested and stained with a monoclonal antibody (mAb) to TCRβ along with empty (control) or PBS-57-loaded CD1d tetramers. Representative dot plots and summary data depict hepatic and splenic i NKT cell frequencies in stressed and control mice. (B) The absolute numbers of i NKT and TCRβ + PBS-57-loaded CD1d tetramer − T conv cells were also calculated. (C) In addition, the percentages of i NKT and T conv cells containing active caspases were determined by flow cytometry. (D) Hepatic i NKT and T conv cells were purified from ≥5 mice that had been either subjected to 2, 6, or 12 h of restraint stress or left undisturbed. After obtaining cDNA, the indicated gene products were amplified by quantitative PCR. Gene expression fold changes in i NKT and T conv cells isolated from stressed mice relative to corresponding cell populations from control animals were calculated using the 2 −(ΔΔCt) method and used to generate a heatmap. (E) Hepatic i NKT and T conv cells were analyzed for their intracellular <t>Bcl-2</t> content. (F) Hepatic T conv cells were enumerated in Nr3c1 fl and Nr3c1 fl Lck cre mice that had been either restrained or left undisturbed. (G) Cohorts of WT B6 mice were given corticosterone (CS) or Veh in drinking water for 21 days before they were sacrificed for their livers and spleens, in which i NKT and T conv cells were enumerated. Each symbol in (A)–(C) and (E)–(G) represents an individual mouse, and error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 by unpaired Student’s t tests. NS, not significant.
    Pe Cy7 Conjugated Anti Mouse Rat Bcl 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe cy7 conjugated anti mouse rat bcl 2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    pe cy7 conjugated anti mouse rat bcl 2 - by Bioz Stars, 2026-04
    86/100 stars

    Images

    1) Product Images from "Chronic stress physically spares but functionally impairs innate-like invariant T cells"

    Article Title: Chronic stress physically spares but functionally impairs innate-like invariant T cells

    Journal: Cell reports

    doi: 10.1016/j.celrep.2021.108979

    (A) WT B6 mice were left undisturbed or restrained for 12 h, after which HMNCs and splenocytes were harvested and stained with a monoclonal antibody (mAb) to TCRβ along with empty (control) or PBS-57-loaded CD1d tetramers. Representative dot plots and summary data depict hepatic and splenic i NKT cell frequencies in stressed and control mice. (B) The absolute numbers of i NKT and TCRβ + PBS-57-loaded CD1d tetramer − T conv cells were also calculated. (C) In addition, the percentages of i NKT and T conv cells containing active caspases were determined by flow cytometry. (D) Hepatic i NKT and T conv cells were purified from ≥5 mice that had been either subjected to 2, 6, or 12 h of restraint stress or left undisturbed. After obtaining cDNA, the indicated gene products were amplified by quantitative PCR. Gene expression fold changes in i NKT and T conv cells isolated from stressed mice relative to corresponding cell populations from control animals were calculated using the 2 −(ΔΔCt) method and used to generate a heatmap. (E) Hepatic i NKT and T conv cells were analyzed for their intracellular Bcl-2 content. (F) Hepatic T conv cells were enumerated in Nr3c1 fl and Nr3c1 fl Lck cre mice that had been either restrained or left undisturbed. (G) Cohorts of WT B6 mice were given corticosterone (CS) or Veh in drinking water for 21 days before they were sacrificed for their livers and spleens, in which i NKT and T conv cells were enumerated. Each symbol in (A)–(C) and (E)–(G) represents an individual mouse, and error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 by unpaired Student’s t tests. NS, not significant.
    Figure Legend Snippet: (A) WT B6 mice were left undisturbed or restrained for 12 h, after which HMNCs and splenocytes were harvested and stained with a monoclonal antibody (mAb) to TCRβ along with empty (control) or PBS-57-loaded CD1d tetramers. Representative dot plots and summary data depict hepatic and splenic i NKT cell frequencies in stressed and control mice. (B) The absolute numbers of i NKT and TCRβ + PBS-57-loaded CD1d tetramer − T conv cells were also calculated. (C) In addition, the percentages of i NKT and T conv cells containing active caspases were determined by flow cytometry. (D) Hepatic i NKT and T conv cells were purified from ≥5 mice that had been either subjected to 2, 6, or 12 h of restraint stress or left undisturbed. After obtaining cDNA, the indicated gene products were amplified by quantitative PCR. Gene expression fold changes in i NKT and T conv cells isolated from stressed mice relative to corresponding cell populations from control animals were calculated using the 2 −(ΔΔCt) method and used to generate a heatmap. (E) Hepatic i NKT and T conv cells were analyzed for their intracellular Bcl-2 content. (F) Hepatic T conv cells were enumerated in Nr3c1 fl and Nr3c1 fl Lck cre mice that had been either restrained or left undisturbed. (G) Cohorts of WT B6 mice were given corticosterone (CS) or Veh in drinking water for 21 days before they were sacrificed for their livers and spleens, in which i NKT and T conv cells were enumerated. Each symbol in (A)–(C) and (E)–(G) represents an individual mouse, and error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 by unpaired Student’s t tests. NS, not significant.

    Techniques Used: Staining, Flow Cytometry, Purification, Amplification, Real-time Polymerase Chain Reaction, Expressing, Isolation


    Figure Legend Snippet:

    Techniques Used: Recombinant, Western Blot, Enzyme-linked Immunosorbent Assay, Staining, In Situ, Selection, Software



    Similar Products

    pe cy7 conjugated anti mouse rat bcl 2  (Thermo Fisher)
    86
    Thermo Fisher pe cy7 conjugated anti mouse rat bcl 2
    (A) WT B6 mice were left undisturbed or restrained for 12 h, after which HMNCs and splenocytes were harvested and stained with a monoclonal antibody (mAb) to TCRβ along with empty (control) or PBS-57-loaded CD1d tetramers. Representative dot plots and summary data depict hepatic and splenic i NKT cell frequencies in stressed and control mice. (B) The absolute numbers of i NKT and TCRβ + PBS-57-loaded CD1d tetramer − T conv cells were also calculated. (C) In addition, the percentages of i NKT and T conv cells containing active caspases were determined by flow cytometry. (D) Hepatic i NKT and T conv cells were purified from ≥5 mice that had been either subjected to 2, 6, or 12 h of restraint stress or left undisturbed. After obtaining cDNA, the indicated gene products were amplified by quantitative PCR. Gene expression fold changes in i NKT and T conv cells isolated from stressed mice relative to corresponding cell populations from control animals were calculated using the 2 −(ΔΔCt) method and used to generate a heatmap. (E) Hepatic i NKT and T conv cells were analyzed for their intracellular <t>Bcl-2</t> content. (F) Hepatic T conv cells were enumerated in Nr3c1 fl and Nr3c1 fl Lck cre mice that had been either restrained or left undisturbed. (G) Cohorts of WT B6 mice were given corticosterone (CS) or Veh in drinking water for 21 days before they were sacrificed for their livers and spleens, in which i NKT and T conv cells were enumerated. Each symbol in (A)–(C) and (E)–(G) represents an individual mouse, and error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 by unpaired Student’s t tests. NS, not significant.
    Pe Cy7 Conjugated Anti Mouse Rat Bcl 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe cy7 conjugated anti mouse rat bcl 2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    pe cy7 conjugated anti mouse rat bcl 2 - by Bioz Stars, 2026-04
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) WT B6 mice were left undisturbed or restrained for 12 h, after which HMNCs and splenocytes were harvested and stained with a monoclonal antibody (mAb) to TCRβ along with empty (control) or PBS-57-loaded CD1d tetramers. Representative dot plots and summary data depict hepatic and splenic i NKT cell frequencies in stressed and control mice. (B) The absolute numbers of i NKT and TCRβ + PBS-57-loaded CD1d tetramer − T conv cells were also calculated. (C) In addition, the percentages of i NKT and T conv cells containing active caspases were determined by flow cytometry. (D) Hepatic i NKT and T conv cells were purified from ≥5 mice that had been either subjected to 2, 6, or 12 h of restraint stress or left undisturbed. After obtaining cDNA, the indicated gene products were amplified by quantitative PCR. Gene expression fold changes in i NKT and T conv cells isolated from stressed mice relative to corresponding cell populations from control animals were calculated using the 2 −(ΔΔCt) method and used to generate a heatmap. (E) Hepatic i NKT and T conv cells were analyzed for their intracellular Bcl-2 content. (F) Hepatic T conv cells were enumerated in Nr3c1 fl and Nr3c1 fl Lck cre mice that had been either restrained or left undisturbed. (G) Cohorts of WT B6 mice were given corticosterone (CS) or Veh in drinking water for 21 days before they were sacrificed for their livers and spleens, in which i NKT and T conv cells were enumerated. Each symbol in (A)–(C) and (E)–(G) represents an individual mouse, and error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 by unpaired Student’s t tests. NS, not significant.

    Journal: Cell reports

    Article Title: Chronic stress physically spares but functionally impairs innate-like invariant T cells

    doi: 10.1016/j.celrep.2021.108979

    Figure Lengend Snippet: (A) WT B6 mice were left undisturbed or restrained for 12 h, after which HMNCs and splenocytes were harvested and stained with a monoclonal antibody (mAb) to TCRβ along with empty (control) or PBS-57-loaded CD1d tetramers. Representative dot plots and summary data depict hepatic and splenic i NKT cell frequencies in stressed and control mice. (B) The absolute numbers of i NKT and TCRβ + PBS-57-loaded CD1d tetramer − T conv cells were also calculated. (C) In addition, the percentages of i NKT and T conv cells containing active caspases were determined by flow cytometry. (D) Hepatic i NKT and T conv cells were purified from ≥5 mice that had been either subjected to 2, 6, or 12 h of restraint stress or left undisturbed. After obtaining cDNA, the indicated gene products were amplified by quantitative PCR. Gene expression fold changes in i NKT and T conv cells isolated from stressed mice relative to corresponding cell populations from control animals were calculated using the 2 −(ΔΔCt) method and used to generate a heatmap. (E) Hepatic i NKT and T conv cells were analyzed for their intracellular Bcl-2 content. (F) Hepatic T conv cells were enumerated in Nr3c1 fl and Nr3c1 fl Lck cre mice that had been either restrained or left undisturbed. (G) Cohorts of WT B6 mice were given corticosterone (CS) or Veh in drinking water for 21 days before they were sacrificed for their livers and spleens, in which i NKT and T conv cells were enumerated. Each symbol in (A)–(C) and (E)–(G) represents an individual mouse, and error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 by unpaired Student’s t tests. NS, not significant.

    Article Snippet: PE-Cy7-conjugated anti-mouse/rat Bcl-2 (Clone 10C4) , Thermo Fisher Scientific , Cat # 25-6992-42; RRID: AB_2573516.

    Techniques: Staining, Flow Cytometry, Purification, Amplification, Real-time Polymerase Chain Reaction, Expressing, Isolation

    Journal: Cell reports

    Article Title: Chronic stress physically spares but functionally impairs innate-like invariant T cells

    doi: 10.1016/j.celrep.2021.108979

    Figure Lengend Snippet:

    Article Snippet: PE-Cy7-conjugated anti-mouse/rat Bcl-2 (Clone 10C4) , Thermo Fisher Scientific , Cat # 25-6992-42; RRID: AB_2573516.

    Techniques: Recombinant, Western Blot, Enzyme-linked Immunosorbent Assay, Staining, In Situ, Selection, Software